Connexins form functional hemichannels in porcine ciliary epithelium
نویسندگان
چکیده
منابع مشابه
Fluid transport across the isolated porcine ciliary epithelium.
PURPOSE To quantify spontaneous fluid transport across the isolated porcine ciliary epithelium and determine its sensitivity to the electrolyte transport inhibitors ouabain and bumetanide, as well as bath Cl(-) and HCO(3)(-) levels. METHODS A complete annulus of ciliary body was mounted in a custom-designed chamber appropriate for quantifying net fluid movement, as well as the transepithelial...
متن کاملSynthesis and assembly of connexins in vitro into homomeric and heteromeric functional gap junction hemichannels.
The biogenesis of connexins and their assembly into functional gap junction hemichannels (connexons) was studied with the use of a cell-free transcription/translation system. Velocity sedimentation on sucrose gradients showed that a small proportion of connexin (Cx) 26 and Cx32 that were co-translationally translocated into microsomes were oligomers of Cx26 and Cx32. Chemical cross-linking stud...
متن کاملElectron microprobe analysis of ouabain-exposed ciliary epithelium: PE-NPE cell couplets form the functional units.
Aqueous humor is secreted by the bilayered ciliary epithelium. Solutes and water enter the pigmented ciliary epithelial (PE) cell layer, cross gap junctions into the nonpigmented ciliary epithelial (NPE) cell layer, and are released into the aqueous humor. Electrical measurements suggest that heptanol reduces transepithelial ion movement by interrupting PE-NPE communication and that gap junctio...
متن کاملFunctional analysis of hemichannels and gap-junctional channels formed by connexins 43 and 46
PURPOSE The gap junctions (GJs) mediating direct cell-cell interaction are formed by clusters of membrane-spanning proteins known as connexins (Cxs). These channels play a key role in signal transmission, and their permeability, time-, and voltage-dependence are governed by the properties of the specific Cxs forming the gap junctions. Retinal pigment epithelium (RPE) cells express Cx43 and Cx46...
متن کاملStudies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.
PURPOSE Bicarbonate transport plays a role in aqueous humor (AH) secretion. The authors examined bicarbonate transport mechanisms and carbonic anhydrase (CA) in porcine nonpigmented ciliary epithelium (NPE). METHODS Cytoplasmic pH (pH(i)) was measured in cultured porcine NPE loaded with BCECF. Anion exchanger (AE), sodium bicarbonate cotransporter (NBC), and CA were examined by RT-PCR and imm...
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ژورنال
عنوان ژورنال: Experimental Eye Research
سال: 2014
ISSN: 0014-4835
DOI: 10.1016/j.exer.2013.11.004